NorthenLab: Exometabolomics linking genomes with environments to understand how webs of microbes sustain biomes

Recent publication highlights new genome engineering technique CRAGE-Duet

In a collaboration with the Yoshikuni lab at JGI and the Dangl lab at University of North Carolina Chapel Hill, Lauren Jabusch of the Northen Lab demonstrated imaging of 9 different colors of fluorescent bacteria within a single EcoFAB. This work is part of the paper “CRAGE-Duet Facilitates Modular Assembly of Biological Systems for Studying Plant–Microbe Interactions.”

Figure 2 from the paper demonstrating imaging of fluorescent proteins in P. simiae inserted using CRAGE-Duet. Fluorescence measurements were taken using a plate reader and viewed under a confocal microscope. ACS Synth. Biol. 2020, 9, 9, 2610-2615. 10.1021/acssynbio.0c00280.

CRAGE, or chassis-independent recombinase-assisted genome engineering, is a method of genome engineering that avoids the pitfalls of plasmid-based tools and allows for the insertion of a genetic construct at a specific location on the chromosome. CRAGE-Duet increases the abilities of CRAGE by enabling the insertion of two constructs in succession. 

Read more about this research.

Brachypodium distachyon growing in a fabricated ecosystem (EcoFAB).