NorthenLab: Exometabolomics linking genomes with environments to understand how webs of microbes sustain biomes

Recent Publications

The most updated list of publications by the Northen Lab is available via google scholar.


Wang, B., Zhao, Z., Jabusch, L.K., Chiniquy, D.M., Ono, K., Conway, J.M., Zhang, S., Wang, G., Robinson, D., Cheng, J., Dangl, J.L., Northen, T.R., and Yoshikuni, Y. CRAGE-Duet facilitates modular assembly of biological systems for studying plant-microbe interactions (2020). ACS Synthetic Biology.

Sasse, J., Kosina, S.M., de Raad, M., Jordan, J.S., Whiting, K., Zhalnina, K., Northen, T.R. Root morphology and exudate availability are shaped by particle size and chemistry in Brachypodium distachyon (2020). Plant Direct, 4(7), e00207.

Ibrahim, M., Fransson, T., Chatterjee, R., Cheah, M.H., Hussein, R., Lassalle, L., Sutherlin, K.D., Young, I.D., Fuller, F.D, Gul, S., Kim, I., Simon, P.S., de Lichtenberg, C., Chernev, P., Bogacz, I., Pham, C.C., Orville, A.M., Saichek, N., Northen, T., Batyuk, A., Carbajo, S., Alonso-Mori, R., Tono, K., Owada, S., Bhowmick, A., Bolotovsky, R., Mendez, D., Moriarty, N.W., Holton, J.M., Dobbek, H., Brewster, A.S., Adams, P.D., Sauter, N.K, Bergmann, U., Zouni, A., Messinger, J., Kern, J., Yachandra, V.K., Yano, J. Untangling the sequence of events during the S2→ S3 transition in photosystem II and implications for the water oxidation mechanism (2020). PNAS, (23) 12624-12635.

Malik, A.A,  Swenson, T., Weihe, C., Morrison, E.W., Martiny, J.B.H., Brodie, E.L., Northen, T.R., Allison, S.D (2020). Drought and plant litter chemistry alter microbial gene expression and metabolite production ISME J.

Zerbe, P., Murphy, K.M., Ewards, J., Louie, K.B., Bowen, B.P., Sundaresan, V., Northen, T.R. (2020). Bioactive Diterpenoids Impact the Composition of the Root-Associated Microbiome in Maize (Zea mays). Research Square.

Doud, D.F.R., Bowers, R.M., Schulz, F., de Raad, M., Deng, K., Tarver, A., Glasgow, E., Muelen, K.V., Fox., B., Deutsch, S., Yoshikuni, Y., Northen, T., Hedlund, B.P., Singer, S.W., Ivanova, N., Woyke, T (2020). Function-driven single-cell genomics uncovers cellulose-degrading bacteria from the rare biosphere. ISME J, 14(3), 659–675.


Morton, J.T., Aksenov, A.A., Nothias, L.F. Foulds, J.R., Quinn, R.A., Badri, M.H., Swenson, T.L., Van Goethem, M.W., Northen, T.R., Vazquez-Baeza, Y., Wang, M., Bokulich, N.A., Watters, A., Song, S.J., Bonneau, R., Dorrestein, P.C., Knight, R. (2019). Learning representations of microbe–metabolite interactions. Nature Methods, 16(12), 1306–1314.

Van Goethem, M.W., Swenson, T.L., Trubl, G., Roux, S., Northen, T.R (2019). Characteristics of Wetting-Induced Bacteriophage Blooms in Biological Soil Crust. mBio, 10(6).

Wang, G., Zhao, Z., Ke, J., Engel, Y., Shi, Y., Robinson, D., Bingol, K., Zhang, Z., Bowen, B., Louie, K., Wang, B., Evans, R., Miyamoto, Y., Cheng, K., Kosina, S., de Raad, M., Silva, L., Luhrs, A., Lubbe, A., Hoyt, D.W., Francavilla, C., Otani, H., Deutsch, S., Washton, N.M., Rubin, E.M., Mouncey, N.J., Visel, A., Northen, T., Cheng, J., Bode, H.B., Yoshikuni, Y. (2019). CRAGE enables rapid activation of biosyntehtic gene clusters in undomesticated bacteria. Nature Microbiology, 4, 2498-2510.

Carmody, R.N., Bisanz, J.E., Bowen, B.P., Maurice, C.F., Lyalina, S., Louie, K.B., Treen, D., Chadaideh, K.S., Rekdal, V.M., Bess, E.N., Spanogiannopoulos, P., Ang, Q.Y., Bauer, K.C., Balon, T.W., Pollard, K.S., Northen, T.R., Turnbaugh, P.J. (2019). Cooking shapes the structure and function of the gut microbiome. Nature Microbiology, 4, 2052-2063.

Brisson, V.L., Schmidt, J.E., Northen, T.R., Vogel, J.P., Gaudin, A.C.M. (2019). Impacts of Maize Domestication and Breeing on Rhizosphere Microbial Community Recruitment from a Nutrient Depleted Agricultural Soil. Scientific Reports, 9, 15611.

Couradeau, E., Sasse, J., Goudeau, D., Nath, N., Hazen, T.C., Bowen, B.P., Chakraborty, R., Malmstrom, R.R., Northen, T.R (2019). Probing the active fraction of soil microbiomes using BONCAT-FACS. Nature communications, 10(1), 1-10.

Chevrette, M.G., Carlos-Shanley, C., Louie, K.B., Bowen, B.P., Northen, T.R, Currie, C.R. (2019). Taxonomic and Metabolic Incongruence in the Ancient Genus StreptomycesFrontiers in Microbiology.

Roth, M. S., Gallaher, S. D., Westcott, D. J., Iwai, M., Louie, K. B., Mueller, M., Walter, A., Foflonker, F., Bowen, B., Ataii, N. Song, J., Chen, J., Blaby-Haas, C., Larabell, C., Auer, M., Northen, T. R., Merchant, S. S., Niyogi, K. K. (2019). Regulation of Oxygenic Photosynthesis during Trophic Transitions in the Green Alga Chromochloris zofingiensis. The Plant Cell, 31(3), 579–601.

Zengler, K., Hofmockel, K., Baliga, N.S., Behie, S.W., Bernstein, H.C., Brown, J.B., Dinneny, J.R., Floge, S.A., Forry, S.P., Hess, M., Jackson, S.A., Jansson, C., Lindemann, S.R., Pett-Ridge, J., Maranas, C., Venturelli, O.S., Wallenstein, M.D., Shank, E.A., and Northen, T.R. (2019). EcoFABs: advancing microbiome science through standardized fabricated ecosystems. Nature Methods, 16, 567-571.

Malik, A. A., Swenson, T., Weihe, C., Morrison, E., Martiny, J. B., Brodie, E. L., Northen, T. R., Allison, S. D. (2019). Physiological adaptations of leaf litter microbial communities to long-term drought. BioRxiv, 631077.

Opgenorth, P., Costello, Z., Okada, T.,  Goyal, G., Chen, Y., Gin, J., Benites, V., de Raad, M., Northen, T. R., Deng, K., Deutsch, S., Baidoo, E. E. K., Petzold, C. J., Hillson, N. J., Martin, H. G., Beller, H. R. (2019). Lessons from two Design-Build-Test-Learn cycles of dodecanol production in Escherichia coli aided by machine learning. ACS Synthetic Biology, acssynbio.9b00020.

Erbilgin, O., Rübel, O., Louie, K. B., Trinh, M., Raad, M. De, Wildish, T., Udwary, D., Hoover, C., Deutsch, S., Northen, T. R., Bowen, B. P. (2019). MAGI: A Method for Metabolite Annotation and Gene Integration. ACS Chemical Biology, 14(4), 704–714.

Sasse, J., Kant, J., Cole, B. J., Klein, A. P., Arsova, B., Schlaepfer, P., Gao, J., Lewald, K., Zhalnina, K., Kosina, S., Bowen, B. P., Treen, D., Vogel, J., Visel, A., Watt, M., Dangl, J. L., Northen, T. R. (2019). Multilab EcoFAB study shows highly reproducible physiology and depletion of soil metabolites by a model grass. New Phytologist, 222(2), 1149–1160.

Diamond, S., Andeer, P. F., Li, Z., Crits-Christoph, A., Burstein, D., Anantharaman, K., Lane, K. R., Thomas, B. C., Pan, C., Northen, T. R., Banfield, J. F. (2019). Mediterranean grassland soil C–N compound turnover is dependent on rainfall and depth, and is mediated by genomically divergent microorganisms. Nature Microbiology.

de Rond, T., Gao, J., Zargar, A., de Raad, M., Cunha, J., Northen, T. R., & Keasling, J. D. (2019). A high‐throughput mass spectrometric enzyme activity assay enabling the discovery of cytochrome P450 biocatalysts. Angewandte Chemie, ange.201901782.

Roux, S., Trubl, G., Goudeau, D., Nath, N., Couradeau, E., Ahlgren, N. A., Zhan, Y., Marsan, D., Chen, F., Fuhrman, J. A., Northen, T. R., Sullivan, M. B., Rich, V. I., Malmstrom, R. R., Eloe-Fadrosh, E. A. (2019). Optimizing de novo genome assembly from PCR-amplified metagenomes. PeerJ, 7, e6902.

Guzmán, G. I., Sandberg, T. E., LaCroix, R. A., Nyerges, Á., Papp, H., de Raad, M., King, Z. A., Hefner, Y., Northen, T. R., Notebaart, R. A., Pal, C., Palsson, B. O., Papp, B., Feist, A. M. (2019). Enzyme promiscuity shapes adaptation to novel growth substrates. Molecular Systems Biology, 15(4), e8462.

Brisson, V., Schmidt, J., Northen, T. R., Vogel, J. P., & Gaudin, A. (2019). A New Method to Correct for Habitat Filtering in Microbial Correlation Networks. Frontiers in Microbiology, 10(March), 1–10.


Porcar, M., Louie, K. B., Kosina, S. M., Van Goethem, M. W., Bowen, B. P., Tanner, K., & Northen, T. R. (2018). Microbial ecology on solar panels in Berkeley, CA, United States. Frontiers in Microbiology, 9(DEC), 1–14.

Gao, J., Sasse, J., Lewald, K. M., Zhalnina, K., Cornmesser, L. T., Duncombe, T. A., Yoshikuni, Y., Vogel, J. P., Firestone, M. K., Northen, T. R. (2018). Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions. Journal of Visualized Experiments, (134), 1–16.

Karaoz, U., Couradeau, E., da Rocha, U. N., Lim, H.-C., Northen, T., Garcia-Pichel, F., & Brodie, E. L. (2018). Large Blooms of Bacillales (Firmicutes) Underlie the Response to Wetting of Cyanobacterial Biocrusts at Various Stages of Maturity . MBio, 9(2), e01366-16.

Zhalnina, K., Zengler, K., Newman, D., & Northen, T. R. (2018). Need for Laboratory Ecosystems To Unravel the Structures. MBio, 9(4), 1–8.

Venturelli, O. S., Carr, A. C., Fisher, G., Hsu, R. H., Lau, R., Bowen, B. P., Hromada, S., Northen, T., Arkin, A. P. (2018). Deciphering microbial interactions in synthetic human gut microbiome communities. Molecular Systems Biology, 14(6), e8157.

Sasse, J., Martinoia, E., & Northen, T. (2018). Feed Your Friends: Do Plant Exudates Shape the Root Microbiome? Trends in Plant Science, 23(1), 25–41.

Deng, K., Zeng, J., Cheng, G., Gao, J., Sale, K. L., Simmons, B. A., Singh, A. K., Adams, P. D., Northen, T. R. (2018). Rapid characterization of the activities of lignin-modifying enzymes based on nanostructure-initiator mass spectrometry (NIMS). Biotechnology for Biofuels, 11(1).

Couradeau, E., Sasse, J., Goudeau, D., Nath, N., Hazen, T. C., Bowen, B. P., Malmstrom, R. R., Northen, T. R. (2018). Study of Oak Ridge soils using BONCAT-FACS-Seq reveals that a large fraction of the soil microbiome is active. BioRxiv, 404087.

Swenson, T. L., Karaoz, U., Swenson, J. M., Bowen, B. P., & Northen, T. R. (2018). Linking soil biology and chemistry in biological soil crust using isolate exometabolomics. Nature Communications, 9(1).

Zhalnina, K., Louie, K. B., Hao, Z., Mansoori, N., Da Rocha, U. N., Shi, S., Cho, H., Karaoz, U., Loque, D., Bowen, B. P., Firestone, M. K., Northen, T. R., Brodie, E. L. (2018). Dynamic root exudate chemistry and microbial substrate preferences drive patterns in rhizosphere microbial community assembly. Nature Microbiology, 3(4), 470–480.

Swenson, T. L., Couradeau, E., Bowen, B. P., De Philippis, R., Rossi, F., Mugnai, G., & Northen, T. R. (2018). A novel method to evaluate nutrient retention by biological soil crust exopolymeric matrix. Plant and Soil, 429(1–2), 53–64.

Iwai, K., Ando, D., Kim, P. W., Gach, P. C., Raje, M., Duncomb, T. A., Heinemann, J. V., Northen, T. R., Martin, H. G., Hillson, N. J., Adamsn, P. D., Singh, A. K. (2018). Automated flow-based/digital microfluidic platform integrated with onsite electroporation process for multiplex genetic engineering applications. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 2018January(January), 1229–1232.

Duncombe, T. A., Raad, M. De, Bowen, B. P., Singh, A. K., & Northen, T. R. (2018). Insulator Nanostructure Desorption Ionization Mass Spectrometry [Article-commentary]. Analytical Chemistry, 90(16), 9657–9661.

Jose, N. A., Lau, R., Swenson, T. L., Klitgord, N., Garcia-Pichel, F., Bowen, B. P., Baran, R., Northen, T. R. (2018). Flux balance modeling to predict bacterial survival during pulsed-activity events. Biogeosciences, 15(7), 2219–2229.

Coates, R. C., Bowen, B. P., Oberortner, E., Thomashow, L., Hadjithomas, M., Zhao, Z., Ke., J., Silva, L., Louie, K., Wang, G., Robinson, D., Tarver, A., Hamilton, M., Lubbe, A., Feltcher, M., Dangl, J., Pati, A., Weller, D., Northen, T. R., Cheng, J., Mouncey, N., Deutsch, S., Yoshikuni, Y. (2018). An integrated workflow for phenazine-modifying enzyme characterization. Journal of Industrial Microbiology and Biotechnology, 45(7), 567–577.

Couradeau, E., Felde, V. J. M. N. L., Parkinson, D., Uteau, D., Rochet, A., Cuellar, C., Winegar, G., Peth, S., Northen, T. R., Garcia-Pichel, F. (2018). In Situ X-Ray Tomography Imaging of Soil Water and Cyanobacteria From Biological Soil Crusts Undergoing Desiccation. Frontiers in Environmental Science, 6(June).


Zhou, A., Lau, R., Baran, R., Ma, J., von Netzer, F., Shi, W., Gorman-Lewis, D., Kempher, M. L., He, Z., Qin, Y., Shi, Z., Zane, G. M., Wu, L., Bowen, B. P., Northen, T. R., Hillesland, K. L., Stahl, D. A., Wall, J. D., Arkin, A. P., Zhou, J. (2017). Key Metabolites and Mechanistic Changes for Salt Tolerance in an Experimentally Evolved Sulfate-Reducing Bacterium, Desulfovibrio vulgaris. mBio, 8(6).

Erbilgin, O., Bowen, B. P., Kosina, S. M., Jenkins, S., Lau, R. K., & Northen, T. R. (2017). Dynamic substrate preferences predict metabolic properties of a simple microbial consortium. BMC Bioinformatics, 18(1), 57.

Sasse, J., Martinoia, E., & Northen, T. R. (2017). Feed Your Friends: Do Plant Exudates Shape the Root Microbiome? Trends in Plant Science, 23(1), 25–41.

Lubbe, A., Bowen, B. P., & Northen, T. (2017). Exometabolomic Analysis of Cross-Feeding Metabolites. Metabolites, 7(4).

Eudes, A., Dutta, T., Deng, K., Jacquet, N., Sinha, A., Benites, V T., Baidoo E E K., Richel A, Sattler S E, Northen T R, Singh S, Simmons B A, Loqué D. (2017). SbCOMT (Bmr12) is involved in the biosynthesis of tricin-lignin in sorghum. PloS One, 12(6), e0178160.

Mondo, S. J., Dannebaum, R. O., Kuo, R. C., Louie, K. B., Bewick, A. J., LaButti, K., Haridas S, Kuo A, Salamov A, Ahrendt S R, Lau R, Bowen B P, Lipzen A, Sullivan W, Andreopoulos B, Clum A,
Lindquist E, Daum C, Northen T R, Kunde Ramamoorthy G, Schmitz R J, Gryganskyi A, Culley D, Magnuson J, James T Y, O’Malley M. A., Starich J. E., Spatafora J. W., Visel A., Grigoriev I. V. (2017). Widespread adenine N6-methylation of active genes in fungi. Nature Genetics, 49(6), 964–968.

Gao, J., Louie, K. B., Steinke, P., Bowen, B. P., Raad, M. de, Zuckermann, R. N., Siuzdak, G., Northen, T. R. (2017). Morphology-Driven Control of Metabolite Selectivity Using Nanostructure-Initiator Mass Spectrometry. Analytical Chemistry, 89(12), 6521–6526.

de Raad, M., de Rond, T., Rübel, O., Keasling, J. D., Northen, T. R., & Bowen, B. P. (2017). OpenMSI Arrayed Analysis Toolkit: Analyzing Spatially Defined Samples Using Mass Spectrometry Imaging. Analytical Chemistry, 89(11), 5818–5823.

Xiong, Y., Wu, V. W., Lubbe, A., Qin, L., Deng, S., Kennedy, M., Bauer, D., Singan, V. R., Barry, K., Northen, T. R., Grigoriev, I. V., Glass, N. L. (2017). A fungal transcription factor essential for starch degradation affects integration of carbon and nitrogen metabolism. PLoS Genetics, 13(5), e1006737.

Baran, R., Lau, R., Bowen, B. P., Diamond, S., Jose, N., Garcia-Pichel, F., & Northen, T. R. (2017). Extensive Turnover of Compatible Solutes in Cyanobacteria Revealed by Deuterium Oxide (D2O) Stable Isotope Probing. ACS Chemical Biology, 12(3), 674–681.

Swenson, T. L., Couradeau, E., Bowen, B. P., De Philippis, R., Rossi, F., Mugnai, G., & Northen, T. R. (2017). A novel method to evaluate nutrient retention by biological soil crust exopolymeric matrix. Plant and Soil.

Jenkins, S., Swenson, T. L., Lau, R., Rocha, A. M., Aaring, A., Hazen, T. C., Chakraborty, R., Northen, T. R. (2017). Construction of Viable Soil Defined Media Using Quantitative Metabolomics Analysis of Soil Metabolites. Frontiers in Microbiology, 8, 2618.

Venturelli, O. S., Carr, A. C., Fisher, G., Hsu, R. H., Lau, R., Bowen, B. P., Northen, T. R., Arkin, A. P. (2017). Deciphering microbial interactions in synthetic human gut microbiome communities. bioRxiv.

Erbilgin, O., Ruebel, O., Louie, K. B., Trinh, M., de Raad, M., Wildish, T., Udwary, D. W., Hoover, C. A., Deutsch, S., Northen, T. R., Bowen, B. P. (2017). MAGI: A Bayesian-like method for metabolite, annotation, and gene integration. bioRxiv.

Heinemann, J., Deng, K., Shih, S. C. C., Gao, J., Adams, P. D., Singh, A. K., and Northen, T. R. (2017) On-chip integration of droplet microfluidics and nanostructure-initiator mass spectrometry for enzyme screening. Lab Chip 17, 323–331.

Yuzawa, S., Deng, K., Wang, G., Baidoo, E. E. K., Northen, T. R., Adams, P. D., Katz, L., and Keasling, J. D. (2017) Comprehensive in Vitro Analysis of Acyltransferase Domain Exchanges in Modular Polyketide Synthases and Its Application for Short-Chain Ketone Production. ACS Synth. Biol. 6, 139–147.

Walker, J. A., Pattathil, S., Bergeman, L. F., Beebe, E. T., Deng, K., Mirzai, M., Northen, T. R., Hahn, M. G., and Fox, B. G. (2017) Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling. Biotechnol. Biofuels 10.


Hiras, J., Wu, Y. W., Deng, K., Nicora, C. D., Aldrich, J. T., Frey, D., Kolinko, S., Robinson, E. W., Jacobs, J. M., Adams, P. D., Northen, T. R., Simmons, B. A., and Singer, S. W. (2016) Comparative community proteomics demonstrates the unexpected importance of actinobacterial glycoside hydrolase family 12 protein for crystalline cellulose hydrolysis. MBio 7, 1–9.

Kosina, S. M., Danielewicz, M. A., Mohammed, M., Ray, J., Suh, Y., Yilmaz, S., Singh, A. K., Arkin, A. P., Deutschbauer, A. M., and Northen, T. R. (2016) Exometabolomics Assisted Design and Validation of Synthetic Obligate Mutualism. ACS Synth. Biol. 5, 569–576.

Lubbe, A., and Northen, T. (2016) Exometabolomics for Linking Soil Carbon to Microbial Communities, in Microbial Metabolomics (Al., D. J. B. et, Ed.), pp 119–145. Springer International Publishing.

Gao J, de Raad M, Bowen BP, Zuckermann RN, Northen TR. Application of black silicon for nanostructure-initiator mass spectrometry. Analytical Chemistry, 2016 88(3) 1625-1630

Walker JA, Takasuka TE, Deng K, Bianchetti CM, Udell HS, Prom BM, Kim H, Adams PD, Northen TR, Fox BG. Multifunctional cellulase catalysis targeted by fusion to different carbohydrate-binding modules. Biotechnol Biofuels. 2015 Dec 21;8:220.

Deng K, Takasuka TE, Bianchetti CM, Bergeman LF, Adams PD, Northen TR, Fox BG. Use of Nanostructure-Initiator Mass Spectrometry to Deduce Selectivity of Reaction in Glycoside Hydrolases. Front Bioeng Biotechnol. 2015 Oct 27;3:165.

de Raad M, Fischer CR, Northen TR. High-throughput platforms for metabolomics. Curr Opin Chem Biol. 2016 Feb;30:7-13.

Deng K, Guenther JM, Gao J, Bowen BP, Tran H, Reyes-Ortiz V, Cheng X, Sathitsuksanoh N, Heins R, Takasuka TE, Bergeman LF, Geertz-Hansen H, Deutsch S, Loqué D, Sale KL, Simmons BA, Adams PD, Singh AK, Fox BG, Northen TR. Development of a High Throughput Platform for Screening Glycoside Hydrolases Based on Oxime-NIMS. Front Bioeng Biotechnol. 2015 Oct 13;3:153.


Baran R, Brodie EL, Mayberry-Lewis J, Hummel E, Nunes Da Rocha U, Chakraborty R, Bowen BP, Karaoz U, Cadillo-Quiroz H, Garcia-Pichel F, Northen TR. Exometabolite niche partitioning among sympatric soil bacteria. Nature Communications, 2015 6(6):8289.

Ceja-Navarro, J. A., F. E. Vega, U. Karaoz, Z. Hao, S. Jenkins, H. C. Lim, P. Kosina, F. Infante, T. R. Northen, and E. L. Brodie. Gut microbiota mediate caffeine detoxification in the primary insect pest of coffee. Nature Communications, 2015, 6:7618.

Yee Ngan CY, Wong CH, Choi C, Yoshinaga Y, Louie K, Jia J, Chen C, Bowen B, Cheng H, Leonelli L, Kuo R, Baran R, JGarcía-Cerdán J,Pratap A, Mei Wang1, Joanne Lim1, Hope Tice1, Daum C, Xu J, Northen TR, Visel A, Bristow J, Niyogi K, Wei CL. Lineage-Specific Chromatin Signatures Reveal a Regulator of Lipid Metabolism in Microalgae. Nature Plants 2015, 1:15107,

Ulisses Nunes da Rocha, Hinsby Cadillo-Quiroz, Ulas Karaoz, Lara Rajeev, Niels Klitgord, Sean Dunn, Viet Truong, Mayra Buenrostro, Benjamin Paul Bowen, Ferran Garcia-Pichel, Aindrila Mukhopadhyay, Trent Russell Northen*, Eoin L Brodie*. Isolation of a significant fraction of non-phototroph diversity from a desert Biological Soil Crust. Frontiers in Microbiology. 2015

Silva L, Northen TR. Exometabolomics and MSI: deconstructing how cells interact to transform their small molecule environment. Current Opinion in Biotechnology. 2015 34, 209-216

Bowen B*, Yao Y, Sun T, Wang T, Ruebel O, Northen T. Analysis of metabolomics datasets with high-performance computing and Metabolite Atlases. Metabolites, 2015, Metabolites 5 (3) 431-442.

Swenson, T. L., Jenkins, S., Bowen, B. P., and Northen, T. R. (2015) Untargeted soil metabolomics methods for analysis of extractable organic matter. Soil Biol. Biochem. 80, 189–198.

Swenson, T. L., Bowen, B. P., Nico, P. S., and Northen, T. R. (2015) Competitive sorption of microbial metabolites on an iron oxide mineral. Soil Biol. Biochem. 90, 34–41.

Kurczy M., Northen T., Trauger, S.A., Siuzdak, G. Nanostructure Imaging Mass Spectrometry: The Role of Fluorocarbons in Metabolite Analysis and the Road to Yoctomole Level Sensitivity. Methods in Mol. Bio. , 2015 1203, 141-149.

Castellanos-Martín, Castillo-Lluva, del Mar Sáez-Freire, Blanco-Gómez, Hontecillas-Prieto, Patino-Alonso, Galindo-Villardon, Pérez del Villar, Carmen Martín-Seisdedos2,6, María Isidoro-Garcia, del Mar Abad-Hernández, Cruz-Hernández, Rodríguez-Sánchez, González-Sarmiento, Alonso-López, De Las Rivas, García-Cenador, Lee, Bowen, Reindl, Northen*, Mao*, Pérez-Losada* Unraveling heterogeneous susceptibility and the evolution of breast cancer by a systems biology approach. Genome Biology 2015 16, 40 2-23.

Sylvie Miquel, Marion Leclerc, rebeca Martin, Florian Chain, Marion Lenoir, Sébastien Raguideau, Sylvie Hudault, Chantal Bridonneau, Trent Northen, Benjamin Bowen, Luis G. Bermúdez-Humarán, Harry Sokol, Muriel Thomas, and Philippe Langella. Identification of metabolic signatures linked to anti-inflammatory effects of Faecalibacterium prausnitzii mBio 2015, 6 (2)

De Rond T, Danielwicz M, Northen T. High throughput screening of enzyme activity with mass spectrometry imaging. Current Opinion in Biotechnology. Current opinion in biotechnology 2015 31, 1-9.


Deng K, Takasuka TE, Heins R, Cheng X, Bergeman LF, Shi J, Aschenbrener R, Deutsch S, Singh S, Sale KL, Simmons BA, Adams PD, Singh AK, Fox BG, Northen TR. Rapid Kinetic Characterization of Glycosyl hydrolases (GHs) based on Oxime Derivatization and Nanostructure-Initiator Mass Spectrometry (NIMS). ACS Chemical Biology 2014, 9(7), 1470-1479. (Cover)

Heins R, Cheng X, Nath S, Deng K, Bowen B; Chivian D, Datta S, Friedland G, D’Haeseleer P, Wu D, Tran-Gyamfi M, Scullin C, Singh S, Shi W, Hamilton M, Bendall M, Sczyrba A, Thompson J, Feldman T, Guenther J, Gladden J, Cheng JF, Adams P, Rubin E, Simmons BA, Sale K, Northen, T, Deutsch S. Phylogenomic Guided Identification of Industrially Relevant GH1 β-Glucosidases Through Coupled DNA Synthesis and Nanostructure-Initiator Mass Spectrometry. ACS Chemical Biology 2014, 19(9), 2082-91.

Vandehey, Nicholas; Brodie, Eoin; Northen, Trent; O’Neil, James. Non-invasive Mapping of Photosynthetic Heterogeneity in Biological Soil Crusts by Positron Emission Tomography: Carbon Fixation. Environmental Science & Technology Letters, 2014, 1(10) 393-398.

Berleman J, Allen S, Danielewicz M, Remis J, Gorur A, Cunha J, Hadi M, Zusman D, Northen T, Witkowska H. Ewa, Manfred Auer, The Lethal Cargo of Myxococcus xanthus Outer Membrane Vesicles, Frontiers in Microbiology 2014, 5, 274, 1-11.


Oliver Rübel, Annette Greiner, Shreyas Cholia, Katherine Louie, E. Wes Bethel, Trent R. Northen, and Benjamin P. Bowen, OpenMSI: High-performance web-based visualization, analysis and management of mass spectrometry imaging data, Analytical Chemistry, 2013 85(21):10354-61.

Baran R, Bowen BP, Price MN, Arkin AP, Deutschbauer AM, Northen TR. Metabolic Footprinting of Mutant Libraries To Map Metabolite Utilization to Genotype. ACS Chemical Biology. 2013, 8 (1), 189-199.

Mosier AC*, Justice NB*, Bowen BP, Baran R, Thomas BC, Northen TR, Banfield JF. Metabolites associated with adaptation of microorganisms to an acidophilic, metal-rich environment identified by stable isotope enabled metabolomics. 2013 Mar 12;4(2):e00484-12 PMCID: PMC3604775.

De Rond T, Peralta-Yahya P, Cheng X, Northen TR, Keasling JD. Versatile synthesis of probes for high-throughput enzyme activity screening. Anal Bioanl Chemistry, 2013 May;405(14):4969-73.

Ryan P. McAndrew, Joshua Park, Wolfgang Reindl, Gregory D. Friedland, Patrik D’haeseleer, Trent Northen, Kenneth L. Sale, Blake A. Simmons, and Paul D. Adams. From soil to structure: a novel dimeric family 3 β-glucosidase isolated from compost using metagenomics. Journal of Biochemistry, 2013,288(21):14985-92.

Louie KB, Bowen BP, McAlhany S, Huang Y, Mao JH, Hellerstein M and Northen TR Kinetic mass spectrometry imaging for in situ kinetic histochemistry, Nature Scientific Reports, 2013 Apr 15;3:1656 PMCID: PMC3625901.

Ho JC, Storm P, Rydström A, Bowen B, Alsin F, Sullivan L, Ambite I, Mok KH, Northen T, Svanborg C. Lipids as tumoricidal components of human alpha-lactalbumin made leathal to tumor cells (HAMLET): unique and shared effects on signaling and death. J Biol Chem. 2013 Jun 14;288(24):17460-71. Epub 2013 Apr 29.

Rajeev L, Nunes da Rocha U, Klitgord N, Luning EG, Fortney J, Axen SP, Shih PM, Bouskill NJ, Bowen BP, Kerfield C, Ferran Garcia-Pichel F, Brodie EL, Northen TR*, Mukhopadhyay, A* Dynamic cyanobacterial response to hydration and dehydration in a desert biological soil crust, ISME Journal, 2013 7, 2178–2191;

Fischer CR, Bowen BP, Pan C, Northen TR, Banfield JF Stable-isotope probing reveals hydrogen isotope fractionation in proteins and lipids in a microbial community are different and species-specific, ACS Chemical Biology 2013, 8 (8), 1755–1763.

Baran, R, Northen, T. Robust Automated Mass Spectra Interpretation and Chemical Formula Calculation Using Mixed Integer Linear Programming. Analytical Chemistry Anal. Chem., 2013, 85 (20), pp 9777–9784.

Baran R, Ivanova N, Jose N, Garcia-Pichel F, Kyrpides N, Gugger M, Northen T. Functional genomics of novel secondary metabolites from diverse cyanobacteria using untargeted metabolomics. Marine Drugs 2013, 11(10), 3617-3631;

Katherine B. Louie, Benjamin P. Bowen, Xiaoliang Cheng, James
Berleman, Romy Chakraborty, Adam Deutschbauer, Adam Arkin and Trent R.
Northen. Replica-extraction-transfer’ for nanostructure-initiator mass spectrometry imaging of acoustically printed bacteria and microbial communities, Analytical Chemistry 2013, 85(22) 10856-62.


Storm, P; Aits, S; Puthia, MK; Urbano, A; Northen, T; Powers, S; Bowen, B; Chao, Y; Reindl, W; Lee, DY; Sullivan, NL; Zhang, L; Trulsson, M; Yang, H; Watson, JD; and Svanborg; C. Conserved features of cancer cells define their sensitivity to HAMLET-induced death; c-Myc and glycolysis. Oncogene 30, 4765-4779.

Deng K,George KW, Reindl W, Keasling JD, Adams PA, Lee TS, Singh AK, Northen TR, Encoding substrates with mass tags to resolve stereospecific reactions using Nimzyme, Rapid Comm. Mass Spectrometry2012 Mar 30;26(6):611-5.

Fischer CR, Wilmes P, Bowen BP, Northen TR, Banfield JF. Deuterium Exchange Metabolomics Identifies N-Methyl lyso Phosphatidylethanolamines As Abundant Lipids in Acidophilic Mixed Microbial Communities. 2012 8 (4) 566-578.

Patti G.J, Tautenhahn R., Fonslow B.R., Cho Y., Deutschbauer A., Arkin A., Northen T., Siuzdak G. Meta-Analysis of Global Metabolomics and Proteomics Data to Link Alterations with Phenotype Spectroscopy (cover article), 2011, 26(3), 151-154

Greving M, Cheng K, Reindl W, Bowen B, Deng D, Louie K, Nyman M, Cohen J, Singh A, Simmons B, Adams P, Siuzdak G, and Northen T Acoustic deposition with NIMS as a high throughput enzyme activity assay, Anal Bioanl Chemistry 2012 May;403(3):707-11. Epub 2012 Mar 10.

Reindl W, Deng K, Cheng X, Singh AK, Simmons B, Adams P, Northen T Nanostructure-Initiator Mass Spectrometry (NIMS) for the Analysis of Enzyme Activities, Curr. Protocols in Chem. Biology, 2012, 4:123‐142.

Lee DY, Platt V, Bowen B, Louie K, Canaria C, McMurray C, Northen T. Resolving brain regions using nanostructure initiator mass spectrometry imaging of phospholipids. Integrative Biology 2012, 4 (6), 693 – 699.

Lee DY, Bowen B, Louie K, Nguyen, D, Parsa S, Huang Y, Mao JH, Northen T. Low-dose ionizing radiation-induced blood plasma metabolic response in a diverse genetic mouse population. Radiation Research, 2012 Dec;178(6):551-5.

Kamennaya N, Ajo-franklin C, Northen T, Jansson C. Biomineralization by Calcifying Cyanobacteria – a Potential Means for Biological Carbon Capture and Storage. Minerals. 2012, 2(4), 338-364;