Development of high throughput enzyme assays to study glycoside hydrolases and lignases
Enzymes play the central role in converting celluloses (hemicelluoses) into simple fermentable sugars and in the deconstruction of lignin. I am developing new assays and high-throughput assay platforms to provide functional annotations for various enzymes and enzyme-variants. This involves the utilization of surface-based nanostructure-initiator mass spectrometry (NIMS), Labman, Biomek automation workstation and microscale liquid handling by acoustic printer. The goal is to provide rapid feedback of the activities of enzymes of interest and help the development of high performance strains.