NorthenLab: Exometabolomics linking genomes with environments to understand how webs of microbes sustain biomes

LignoChip presented at 2022 ASM Microbe conference

Northen Lab researcher Jenny Onley attended the 2022 ASM Microbe conference this past June, presenting the poster “High-Throughput and Bond-Specific Analysis of Lignocellulose Deconstructing Enzymes with LignoChip.” This technology was developed by a team of scientists including other lab members Kai Deng, Noel Ha, Markus de Raad, and Trent Northen. 

Lignocellulosic biomass (lignin, cellulose, and hemicellulose) is an abundant source of carbon and has significant potential for biofuels and other important chemicals. Characterizing lignocellulosic enzymes is important for finding enzymes that can effectively transform this biomass. The LignoChip is a combination of nanostructure-initiated mass spectrometry (NIMS), droplet microfluidics, and mass spectrometry imaging (MSI). The system is capable of testing many different combinations of enzymes and substrates. 

Reference: 

Onley, J.R., K. Deng, N.S. Ha, M. de Raad, L.T.M. Pham, P. Wolski, N. Kuch, B. G. Fox, K. L. Sale, P. D. Adams, T. R. Northen. High-Throughput and Bond-Specific Analysis of Lignocellulose Deconstructing Enzymes with LignoChip. American Society for Microbiology Microbe Meeting, Washington D.C., USA, June 9-13, 2022.