NorthenLab: Exometabolomics linking genomes with environments to understand how webs of microbes sustain biomes

Metabolomics identifies PCA and PDC production

JGI Metabolomics researchers Andrea Kuftin, Katherine Louie, and Trent Northen contributed to the paper “Development of platforms for functional characterization and production of phenazines using a multi-chassis approach via CRAGE,” which utilized metabolomics to analyze PCA (phenazine-1-carboxylic acid) and PDC (phenazine-1,6-dicarboxylic acid) from bacterial cultures. 

As previously highlighted, CRAGE (or chassis-independent recombinase-assisted genome engineering) enables insertion of genetic material at specific locations along a genome. This latest research aimed to develop a strain capable of high PDC production using CRAGE, relying on metabolomics to help determine PCA and PDC production in the genetically modified organisms. Potential derivatives of PCA and PDC were also identified in multiple strains after untargeted metabolomic analyses. 

Image: MSMS spectra of PCA, PDC, phenazine, pyrocyanin, and phenazine-1-carboxamide.

To learn more, check out the original paper.